Methylene Violet
Methylene Violet (Bernthsen)
CAS: 2516-05-4
Molecular Formula: C14H12N2OS
Methylene Violet - Names and Identifiers
| Name | Methylene Violet (Bernthsen) |
| Synonyms | CI 52041 C.I. 52041 Methylene Violet Methylenevioletdye SAFRANIN 6B BLUISH Methylene Violet (Bernthsen) METHYLENE VIOLET (BERNTHSEN) CERTIFIED 7-(dimethylamino)-3H-phenothiazin-3-one Methylene Violet, certified, Bernthsen, pure Methylene Violet (Bernthsen), high purity biological stain, pure METHYLENE VIOLET (BERNTHSEN), CERTIFIED BY THE BIOLOGICAL STAIN COMMISSION |
| CAS | 2516-05-4 |
| EINECS | 219-733-4 |
| InChI | InChI=1/C14H12N2OS/c1-16(2)9-3-5-11-13(7-9)18-14-8-10(17)4-6-12(14)15-11/h3-8H,1-2H3 |
Methylene Violet - Physico-chemical Properties
| Molecular Formula | C14H12N2OS |
| Molar Mass | 256.32 |
| Density | 1.29±0.1 g/cm3(Predicted) |
| Melting Point | 216 °C |
| Boling Point | 429.6±45.0 °C(Predicted) |
| Flash Point | 213.6°C |
| Solubility | Insoluble in water, soluble in ethanol |
| Vapor Presure | 1.38E-07mmHg at 25°C |
| Appearance | Powder |
| Color | Dark green |
| Maximum wavelength(λmax) | 580 nm |
| pKa | 5.10±0.20(Predicted) |
| Refractive Index | 1.678 |
Methylene Violet - Risk and Safety
| Safety Description | 24/25 - Avoid contact with skin and eyes. |
| HS Code | 32041900 |
Methylene Violet - Standard
Authoritative Data Verified Data
This product is a mixture of tetramethyl pararosaniline chloride, pentamethyl pararosaniline chloride and hexamethyl pararosaniline chloride.
Last Update：2024-01-02 23:10:35
Methylene Violet - Trait
Authoritative Data Verified Data
- This product is dark green-purple granular powder or green-purple fragments with metallic luster; The odor is minimal.
- This product is dissolved in ethanol or chloroform, slightly soluble in water, insoluble in ether.
Last Update：2022-01-01 11:38:31
Methylene Violet - Differential diagnosis
Authoritative Data Verified Data
- take about 1 mg of this product and spread it on the liquid surface of 1 ml of sulfuric acid to dissolve it into an orange-yellow or brown-red solution. Dilute it with water to turn brown, gradually turn green, and finally turn blue.
- take about 20mg of this product, Add 10ml of water to dissolve, add 5 drops of hydrochloric acid, shake well; Take 5ml of solution, add tannic acid solution Dropwise, that is, generate dark blue precipitate.
- take the remaining solution under identification (2), add about 0.5g of zinc powder, heat, I .e., fade; Take 1 drop, put one drop of ammonia test solution on the filter paper, and the interface will appear blue when the two drops diffuse and contact.
Last Update：2022-01-01 11:38:32
Methylene Violet - Exam
Authoritative Data Verified Data
insolubles in ethanol
take this product l. After heating and refluxing for 15 minutes, filter with a vertical melting glass crucible of constant weight at 105 ° C., and the filter residue is washed with hot ethanol until the washing solution is no longer Pansy, after drying at 105°C to constant weight, the remaining residue should not exceed 0.5%.
loss on drying
take this product, dry to constant weight at 105°C, weight loss shall not exceed 7.5% (General rule 0831).
ignition residue
take 0.5g of this product and check it according to law (General rule 0841). The remaining residue shall not exceed 1.5%.
Heavy metals
The residue left under the item of ignition residue shall not contain more than 0821 parts per million of heavy metal after examination by law (General Chapter, Law II).
arsenic salt
take 0.2g of this product, add 0.5g of calcium hydroxide, mix, add a small amount of water, stir evenly, after drying, burn with small fire to make charring, then at 500~600°C, it is completely Ash, cool, add 5ml of hydrochloric acid and 23ml of water to dissolve, and check according to law (General Principles 0822 The first law), should comply with the regulations (0.001% )
Last Update：2022-01-01 11:38:33
Methylene Violet - Category
Authoritative Data Verified Data
Disinfection antiseptic.
Last Update：2022-01-01 11:38:33
Methylene Violet - Storage
Authoritative Data Verified Data
light shielding, sealed storage.
Last Update：2022-01-01 11:38:33
Methylene Violet - Methylrosaniline Chloride Solution
Authoritative Data Verified Data
This product contains 0.85% ~ 1.05%(g/ml) of Methyl violet.
preparation method
take methyl violet, add an appropriate amount of ethanol to dissolve, add an appropriate amount of water to make 1000ml, and filter to obtain.
trait
This product is purple liquid.
identification
- take 5ml of this product, add 2 drops of hydrochloric acid, and add tannic acid solution Dropwise to generate dark blue precipitate.
- take 2ml of this product, put it on a water bath to dry, take a little residue, spread it on the liquid surface of 1 ml sulfuric acid, and dissolve it into orange yellow or brown red solution, and add water to dilute it, it turns brown, gradually green, and finally blue.
examination
The amount of this product, according to the law inspection (General 0942), should comply with the provisions.
Content determination
Take 10ml of this product for precise measurement, put it in an evaporation dish with constant weight at 105°C, put it on a water bath, and then dry it at 105°C to constant weight, and calculate it to obtain it.
category
The same as that of Methyl violet.
storage
light shielding, sealed storage.
Last Update：2022-01-01 11:38:34
Methylene Violet - Introduction
Nature:
Methylene Violet (Bernthsen) is a purple crystalline solid. It is soluble in water and alcohol solvents, but less soluble. The compound has good thermal stability and light resistance.
Use:
Methylene Violet (Bernthsen) is commonly used in the laboratory to stain biological materials such as cells, bacteria and tissue sections. It can also be used as a dye in the textile, leather and pharmaceutical industries.
Preparation Method:
Methylene Violet (Bernthsen) has many preparation methods, one of which is commonly used by the reaction of dimethylaniline and formaldehyde. The particular preparation involves a series of oxidation, amination and chlorination reaction steps.
Safety Information:
The following security considerations apply when using methyllene Violet (Bernthsen):
1. Methylene Violet (Bernthsen) is toxic, contact with skin, eyes or inhalation of its dust may cause irritation and allergic reactions. Therefore, appropriate personal protective equipment such as gloves, goggles and protective masks should be worn during use.
2. Avoid prolonged exposure to Methylene Violet (Bernthsen) because it may cause damage to the human body.
3. When storing and handling the Methylene Violet (Bernthsen), follow the relevant safety procedures, and ensure that it is properly sealed and stored in a dry place away from light.
4. When disposing of Methylene Violet (Bernthsen) waste, dispose of it safely in accordance with local regulations.
Last Update：2024-04-09 20:48:19
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Spot supply
Product Name: Alimemazine Impurity 4 Visit Supplier Webpage Request for quotationCAS: 2516-05-4
Tel: 0714-3999186
Email: 2853786052@qq.com
Mobile: 86+15671228036
QQ: 2853786052
Wechat: 15671228036
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